Methods & Expertise
Start with a question.
Connect the methods.
We connect clinical questions with cellular, tissue and data-based evidence. These approaches are represented in our published experiments and collaborations.
Cell models & mechanistic testing
Gene perturbation in UV- and oxidative-stress models of human dermal fibroblasts links candidate pathways to senescence and molecular readouts.
Gene overexpression / knockdownSA-β-galqPCR / immunoblottingSASP-related expression
02 / Published experienceTranscriptome integration & cell states
Integration of study-generated bulk and single-cell RNA-seq with public datasets identifies candidates and cell states. Trajectory, regulatory-network and cell-interaction inference generates hypotheses for functional testing.
Differential expressionPathway analysisPublic scRNA-seq reanalysisCross-dataset validationPAGA / pseudotimepySCENICCellPhoneDB
03 / Published experienceImmune phenotype & function
Collaborative high-dimensional immune profiling is paired with functional assays and clinical-severity analyses to investigate the meaning of distinct cell populations.
CyTOFviSNE / FlowSOMFlow cytometryTreg suppression assay
04 / Published experienceTissue-level validation
Immunohistochemistry, immunofluorescence and quantification connect findings from cells and data with evidence in human skin.
ImmunohistochemistryImmunofluorescenceImage quantification
05 / Published experienceMultidisciplinary skin–brain studies
Collaborative studies connect mouse skin-exposure models with LC–MS, pharmacological perturbation, behavior, electrophysiology and tissue analysis.
LC–MSReceptor antagonismBehavioral testingHippocampal electrophysiology
06 / Published experienceSkin imaging & data-analysis collaborations
We collaborate with optical and engineering researchers on skin-image interpretation and quantitative assessment. Image processing and AI-based analyses are used where relevant to the research question, with attention to clinical meaning and applicability.
Skin imagingImage processingQuantitative assessment
07 / Published experienceMedical devices, EBD & outcome assessment
Our clinical study used 3D imaging to quantify facial-pore changes after combined microfocused ultrasound and intradermal incobotulinumtoxin-A, connecting treatment conditions with objective outcome assessment.
Microfocused ultrasound3D imaging / pore quantificationClinical outcome assessment
Specialized platforms and analyses include work with collaborators and research facilities. Experimental design, analytical scope and contributions are discussed for each project.
A question we could test together? ↗